Fig 2: Impact of HIV-1 infection on OAS expression. Pericytes were either mock-infected or infected with 60 ng/mL HIV-1 p24 for 24, 48, or 72 h and mRNA and protein expression of OAS1 (A), OAS2 (B), OAS3 (C), and OASL (D) was measured by q-PCR and immunoblotting, respectively. GAPDH was used as a housekeeping gene and loading control. Values are mean ± SEM. ****p<0.0001, ***p=0.0002, **p=0.003, *p<0.05, n=4–6 independent samples per group

Fig 3: Ocln upregulation enhances OAS expression in human brain pericytes. Pericytes were transfected with ocln overexpression vector PCMV3-OCLN or with PCMV3 control vector, and the expression of mRNA and protein for ocln (A), OAS1 (B), OAS2 (C), OAS3 (D), and OASL (E) was evaluated by q-PCR and immunoblotting respectively. GAPDH was used as a housekeeping gene and loading control. Values are mean ± SEM. ****p<0.0001, ***p=0.0002, **p=0.003, *p<0.05, n=4–6 independent samples per group

Fig 4: Ocln upregulation enhances OAS expression in human brain pericytes.Pericytes were transfected with ocln overexpression vector PCMV3-OCLN or with PCMV3 control vector, and the expression of mRNA and protein for ocln (A) OAS1 (B) OAS2 (C) OAS3 (D) and OASL (E) was evaluated by q-PCR and immunoblotting respectively. GAPDH was used as a housekeeping gene and loading control. Values are mean ± SEM. ****p < 0.0001, ***p = 0.0002, **p = 0.003, *p < 0.05, n = 4–6 independent samples per group.

Fig 5: The OAS family members regulate each other’s expression.Pericytes were transfected as in Figure 4, and mRNA and protein levels of OAS1 (A) OAS2 (B) OAS3 (C) and OASL (D) was evaluated by q-PCR and immunoblotting, respectively. GAPDH was used as a housekeeping gene and loading control. Values are mean ± SEM. ****p < 0.0001, ***p = 0.0002, **p = 0.003, *p < 0.05, n = 4–6 independent samples per group.